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非小細胞肺癌中垂體瘤轉化基因的表達及臨床意義

摘要:目的:研究垂體瘤轉化基因在非小細胞肺癌組織、肺良性病變組織和正常支氣管黏膜上皮組織中的表達, 初步探討其與非小細胞肺癌發生發展,侵襲轉移的關系。方法:(1)用SP免疫組化法檢測76例臨床石蠟組織標本(44例非小細胞肺癌、20例肺良性病變組織和12例正常支氣管黏膜上皮組織)中的PTTG蛋白的表達。(2)用RTPCR法分析PTTG mRNA在不同性質肺組織中的表達情況。〖HTH〗結果〖HTSS〗:(1)PTTG蛋白在不同性質肺組織中的表達差別具有明顯差異;在TNM分期、淋巴結轉移組間差別有統計學意義。(2)PTTG mRNA在不同性質肺組織中的表達差別具有明顯差異(Plt;0.001);在TNM分期、淋巴結轉移組間差別有統計學意義。結論：細胞肺癌的發生發展及轉移有關。Abstract: Objective: To investigate the association between PTTG expression and biological behaviors of human nonsmall cell lung cancer (NSCLC).Methods:SP immunohistochemistry was used to detect the expression of PTTG in 76 cases of paraffinembedded specimens (including 44 surgical specimens from NSCLC patients, 20 pneumonic benign lesion and 19 normal bronchial epithelium).Realtime RTPCR was performed to detect PTTGm RNA expression in 44 cases of fresh carcinoma and corresponding adjacent normal mucosa specimens.Results: The expression levels of PTTG was significantly different between normal mucosa and adenoma tissues.There were statistical relationships between their expressions and TNM stage, lymphnode metastasis. Conclusion: PTTG may play an important role in carcinogenesis、development and metastasis of human nonsmall cell lung cancer.